Determination of Domain Structure of Proteins from X-Ray Solution Scattering
نویسندگان
چکیده
منابع مشابه
Determination of domain structure of proteins from X-ray solution scattering.
An ab initio method for building structural models of proteins from x-ray solution scattering data is presented. Simulated annealing is employed to find a chain-compatible spatial distribution of dummy residues which fits the experimental scattering pattern up to a resolution of 0.5 nm. The efficiency of the method is illustrated by the ab initio reconstruction of models of several proteins, wi...
متن کاملNew methods for domain structure determination of proteins from solution scattering data
Author(s) of this paper may load this reprint on their own web site provided that this cover page is retained. Republication of this article or its storage in electronic databases or the like is not permitted without prior permission in writing from the IUCr. New approaches for domain structure determination of macromolecules from X-ray solution scattering are presented. An ab initio method for...
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One of the most important overall parameters, which can be derived from smallangle X-ray scattering (SAXS) experiments on macromolecular solutions is the molecular mass (MM) of the solute. In particular, for a monodisperse protein solution, MM of the solute is calculated from the extrapolated scattering intensity at zero angle I(0). Assessing MM by SAXS provides valuable information about the o...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2001
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(01)76260-1